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Electrophoresis of samples obtained using a basic alkaline plasmid purification procedure demonstrated the presence of endogenous plasmids that had not integrated into the host genome (data not shown).
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Mononuclear cells (MNC) were isolated for genetic damage analysis using the basic alkaline comet assay.
Plasmid DNA was isolated using a standard alkaline lysis protocol.
DNA extractions on individuals from the laboratory colonies were performed using a simple alkaline method [30].
BAC DNA was isolated using a modified alkaline lysis method [23].
GR AF1 activity was determined using a secreted alkaline phosphatase (SEAP) assay as described [7].
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