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Total RNA from A10 cells was isolated using a Trizol procedure (Invitrogen, Carlsbuel, CA).
Total RNA was extracted from whole fry using a Trizol procedure and purified with a phenol: chloroform extraction followed by an ethanol precipitation.
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Total RNA was obtained from the cell lines using a TRIzol kit (Gibco BRL, Grand Island, NY, USA) according to the procedure recommended by the supplier.
Total RNA was extracted from 25 female heads per replicate using a Trizol-Chloroform-based procedure (Invitrogen) and subsequently treated with DNAse I (Ambion).
Total RNA was isolated from newly fertilized siliques of six weeks seedlings using a TRIzol kit (Invitrogen).
Total RNA was extracted using a TRIzol kit according to the manufacturer's instructions (Invitrogen, http://www.invitrogen.com/).
Total RNA was isolated using a Trizol protocol (Invitrogen).
Total RNA was isolated using a Trizol kit (Invitrogen, USA).
RNA was extracted using a Trizol-based protocol.
To summarise, the following principle procedures were followed: Total RNA was isolated from freeze-dried tissues using the Trizol procedure (Chomczynski and Sacchi, 1987) incorporating a final LiCl2 precipitation.
Total RNA was extracted using the Trizol procedure (GIBCO-BRL).
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