Sentence examples for using a TaqMan probe from inspiring English sources

Genotyping of TNIP1 rs7708392 was carried out using the TaqMan genotyping assay (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's instructions, using a TaqMan probe C__29349759_10.

A one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) using a TaqMan probe to quantitatively detect spring viremia of carp virus (SVCV) is described.

The bteA IS481a-promoter transcript (P2 transcript) was determined using a TaqMan probe IS481-bteAA probe).

The results obtained using a Taqman probe designed against other regions of luc gene sequence were similar (data not shown).

To validate the results using these primers, real-time RT-PCR using a Taqman probe designed by Applied Biosystems was carried out as previously indicated [43].

Real-time PCR of MGMT was performed on the ABI PRISM 7900 HT detection system using a taqman probe (Hs00172470_m1, Applied Biosystems) and taqman reagents under default conditions: 95°C for 10 minutes, 40 cycles of 95°C for 15 seconds, and 60°C for 1 minute with human beta-glucuronidase (hGUS) as endogenous control.

RNA was extracted as before and RRT-PCR performed using a Taqman probes system as described by EU Community Reference Laboratory protocols [45].

For this we used a Taqman probe spanning exons 2 and 3 (ExJ2-3) of GPR110, which measures both isoforms 1 and 2; and GUSB, a moderately expressed gene, as an endogenous control gene.

In this paper, we report on the development of real-time detection of Leptospira spp. using a TaqMan fluorogenic probe.

Real-time PCR was performed using cDNA, a TaqMan probe, and Real-time PCR master mix (QPK-101, TOYOBO, Osaka) using the Taqman assay protocol.

Real-time PCR differs from conventional methods in that the amplified product is measured after each thermal cycle using either a TaqMan probe [ 21] or an intercalating chemical like SYBR Green [ 22] to generate a fluorescent signal.