Sentence examples for using a Superscript cdna from inspiring English sources

Total RNA (2 μg) from samples was reverse transcribed using a SuperScript cDNA synthesis kit (Invitrogen).

WNT10A cDNA was constructed by PCR using a superscript cDNA library (Invitrogen) (Table S2).

Using a poly-dT primer incorporating a T7 promoter, double-stranded cDNA was synthesized from 10 μg total RNA using a Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA).

Double stranded cDNA was synthesized using a Superscript cDNA synthesis kit (Invitrogen) with random hexamer primers at a concentration of 5 μM.

The first-strand cDNA was synthesized from 1.0  μg of Poly A+ mRNA using a Superscript cDNA synthesis kit (Invitrogen Corp., Carlsbad, CA, USA) according to the manufacturer's instructions.

Briefly, isolated total RNA was converted to double-stranded cDNA using a Superscript cDNA Synthesis Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions, except that a specific primer (BSL-18E) was used [ 28].

cDNA was synthesized using a SuperScript VILO cDNA synthesis kit (Invitrogen) SYBR-Green real-time PCR (Applied Biosystems, Foster City, CA) was performed on cDNA prepared from each sample using Platinum SYBR-Green qPCR Super-Mix-UDG (Invitrogen) and 0.5 μM each primer (Table  1).

In brief, Alexa 555- or 647-labelled cDNA was produced from the RNA, using a Superscript direct cDNA labelling system (Invitrogen) and Alexa 555 and 647 dUTP label mix.

To measure tumour PROK1 and PROK2 content, ∼60 200 ng of extracted RNA was first reverse transcribed into cDNA in a 20- μl reaction using a Superscript VILO cDNA synthesis kit according to the manufacturer's protocol (Invitrogen, Carlsbad, CA, USA).

After DNase treatment, first strand cDNAs were synthesized from total RNA using a SuperScript VILO cDNA Synthesis Kit (Invitrogen, Carlsbad, CA, USA).

Amino-modified cDNAs were synthesized from 20 μg of pooled RNAs by using a SuperScript indirect cDNA labeling kit (Invitrogen) and labeled with Cy3 or Cy5 fluorescent dyes (Amersham Pharmacia, Piscataway, NJ, USA).