Sentence examples for using a SuperSMART cdna from inspiring English sources
SSH libraries were constructed using a SuperSMART cDNA Synthesis kit (Clontech) and a Clontech PCR-Select cDNA Subtraction kit (Clontech).
Double stranded cDNAs were constructed using SuperSMART cDNA Synthesis Kit (BD Bioscience Clontech K1054-1) as described by Bassett et al. [ 42]. cDNA-AFLP experiments were conducted using the Licor procedure (Li-Cor, ALFP IRDey 800 830-061944).
Poly-A RNA was isolated from ∼1 μg total RNA, and reverse-transcribed into complementary DNA (cDNA) using a random priming cDNA Synthesis kit (Roche).The cDNA was converted into a sequencing library for 454 sequencing using the GS FLX Titanium Rapid Library Preparation kit and sequenced on the Roche 454 platform.
Total RNA was extracted using an RNA extraction solution (BioAssay Co., Daejeon, Korea) and reverse transcribed to cDNA using a 1st Strand cDNA synthesis kit (BioAssay Co ., according to the manufacturer's protocol.
RNA was quantified with a spectrophotometer, and 1 μg of isolated RNA was reverse-transcribed into cDNA using a first-strand cDNA synthesis kit (Promega Corp., Madison, WI, USA) following the manufacturer's protocol.
Total RNA was extracted from cells using Trizol (Invitrogen) and was transcribed to cDNA by using a high capacity cDNA reverse transcription kit (Applied Biosystems, CA, USA).
Total RNA (2 μg) from samples was reverse transcribed using a SuperScript cDNA synthesis kit (Invitrogen).
RNA was purified using TRI Reagent (Sigma) and complementary DNA (cDNA) prepared using a SensiFast cDNA synthesis kit (Bioline) according to the manufacturers' instructions and qRT-PCR performed using a LightCycler 480 (Roche).
RNA was purified using TRI Reagent (Sigma) and cDNA prepared using a SensiFast cDNA synthesis kit (Bioline) according to the manufacturers' instructions and qRT-PCR performed using a LightCycler 480 (Roche) with oligonucleotides in Supplemetary Table 1.
ODC mRNA levels were quantified using a cloned cDNA probe.
The cDNA was synthesized using a 1st-STRAND cDNA Synthesis Kit (Fermentas, Pittsburgh PA, USA).
