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At 72 post-transduction, RNA was isolated from three independent cell culture preparations, and cDNA was synthesized using a Strand cDNA Synthesis Kit (Takara, Japan).
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5 μg of each RNA sample was converted into first strand cDNA using a First Strand cDNA Synthesis Kit (Fermentas, Burlington, Canada) using random hexamer primers following the manufacturer's instructions.
Then, precisely 2.5 μg from each of the extracted RNA samples was used for first-strand cDNA synthesis using a First Strand cDNA Synthesis kit (Amersham, Buckinghamshire, UK) according to the manufacturer's instructions.
Single-strand cDNA was obtained using a First Strand cDNA Synthesis Kit (Takara, Dalian, China).
Total RNA was extracted using an RNA extraction solution (BioAssay Co., Daejeon, Korea) and reverse transcribed to cDNA using a 1st Strand cDNA synthesis kit (BioAssay Co ., according to the manufacturer's protocol.
Five µg of total RNA was used to produce cDNA using a First Strand cDNA Synthesis Kit (SuperArray).
Aliquots (∼1 µg) of the total RNA extracts were then reverse-transcribed into cDNA using a First Strand cDNA Synthesis Kit (Amersham Pharmacia Biotech, Oakville, ON, Canada).
Product was then reverse transcribed into cDNA using a first strand cDNA synthesis kit (SABiosciences).
One microgram of RNA was reverse transcribed into cDNA using a first strand cDNA synthesis kit (Thermo Scientific).
Then 1 µg RNA was converted to cDNA using a First Strand cDNA Synthesis Kit (Roche, Indianapolis, IN).
One microgram of the RNA was used to generate complementary DNA (cDNA) using a first strand cDNA synthesis kit from Applied Biosystems.
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