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Images were captured using a Slow Scan CCD camera (Proscane) and EsiVision Pro 3.2 software (Soft Imaging Systems GmbH).
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To increase resolution, we used a slow scan speed, a very small z slice interval and a small pinhole optimized to 1 airy unit.
Images were collected using a slow-scan CCD camera with 4096×4096 pixels at a resolution of 0.71 nm per pixel.
Micrographs were recorded under low-dose conditions using a slow-scan CCD camera (Gatan, model 794).
Using a slow cooker.
For image acquisition, EELS and ESI, a slow scan dual speed CCD camera "Sharpeye" (Tröndle, Moorenweis, Germany) was used, operated by iTEM software (Soft Image System, Münster, Germany).
VLPs were imaged at 40,000x indicated magnification using a 4k×4k slow-scan CCD camera (UltraScan 895, GATAN, Inc., Pleasanton, CA) using a low-dose imaging procedure.
Axial motion artifacts are commonly corrected by using cross-correlation methods and rigid translations of each B-scan [ 24, 26] or through acquisition of orthogonal B-scans to aid alignment by providing a slow scan axis reference frame with no axial motion [ 27].
Severe ablation occurred when the average power at 20 kHz was set the same as that at 500 kHz because the peak power at 20 kHz was 25 times higher than that at 500 kHz; thus, the average power at 20 kHz was set to be 20% of the average power at 500 kHz, and the total exposure energy was compensated by using an 80% slower scan speed instead.
Electronic micrographs were captured with a slow scan CCD camera (S.I.S. Megaview III).
Use a slow feeder.
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