Sentence examples for using a RNeasy mini from inspiring English sources
Total RNA from transfected or infected cells were harvested by using a RNeasy mini kit (Qiagen).
Total RNA was prepared using a RNeasy mini kit (Qiagen) and treated with DNase I, Amp Grade (InVitrogen) prior to cDNA synthesis.
Total RNAs from the 3D constructs were extracted using a RNeasy mini kit (Qiagen, Valencia, California).
Total RNA preparations were DNaseI treated (Ambion, TX) and purified using a RNeasy mini kit (Qiagen, CA).
Total RNA was extracted from hippocampal samples using a RNeasy mini kit (Qiagen) with on column DNase treatment (Qiagen).
Extraction of total RNA from pituitaries and micropunches was performed using a RNeasy mini kit (Quiagen) according to the manufacturer′s guidelines.
To verify the transcription of AaIT gene in Bb-AaIT, the total RNAs were extracted from the CDA culture supernatant or infected mosquitoes using an RNeasy mini plant kit (Qiagen, Duesseldorf, GER), and they were subjected to reverse transcription with random primers and then PCR with the primers RT-AaIT-F and RT-AaIT-R (5′-CTTTCTTCGGTTCTCGCCCT-3′ and 5′-CCTTATCGGCGTAGTGGACC-3′, respectively).
Total RNA was extracted using an RNeasy mini kit (Qiagen) and eluted with 35 µl of RNAase-free water.
Total RNA was extracted from cells using an RNeasy mini kit (Qiagen, USA) and following manufacturer's instructions.
Total RNAs were isolated from both samples using an RNeasy mini kit (Qiagen) after 10-hour infection.
To compare the transcript levels, total RNA from neurons was isolated using an RNeasy mini kit (Qiagen, Valencia, CA, USA).
