Sentence examples for using a Quantitect cdna from inspiring English sources

Extracted RNA was converted into cDNA by reverse transcription of 500 ng total RNA using a Quantitect cDNA synthesis kit (Qiagen).

Total RNA (1 μg) was converted into cDNA using a Quantitect Reverse Transcription kit (Roche, Mannheim, Germany) according to the manufacturer's instructions.

Total RNA was extracted using an RNeasy Micro Kit (Qiagen, Hilden, Germany), and equal amounts of RNA were reverse transcribed into cDNA using a QuantiTect Reverse Transcription Kit (Qiagen).

Following manufacturer's instructions, one microgram of total RNA was used to make first strand cDNA using a Quantitect Reverse Transcription kit (Qiagen) that incorporates genomic DNA removal prior to reverse transcription.

Total RNA (100-200 ng) was employed for cDNA synthesis using a QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany).

Cells were pelleted for RNA isolation using an RNeasy Mini Kit (Qiagen, Hilden, Germany) and cDNA synthesis using a QuantiTect Reverse Transcription Kit (Qiagen).

cDNA was generated using a Quantitect reverse transcription kit for RT-PCR (Qiagen).

cDNA was synthesized using a QuantiTect Reverse Transcription Kit (Qiagen, Germany).

cDNA was made using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA).

cDNA was synthesized using a QuantiTect Reverse Transcription Kit (Qiagen K.K., Tokyo, Japan).

cDNA was generated using a QuantiTect Reverse transcription kit (Qiagen) or alternatively using oligo dT primers and Superscript III reverse transcriptase (Invitrogen).