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Cells were suspended at 10 cells/μl in PBS containing 2% FBS (HyClone, Logan, UT, USA) at pH 7.2 and fractionated into positive and negative populations by sequentially placing the cells through two LS columns using a QuadroMACs magnet (Miltenyi Biotec).
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Then, an external magnetic field was applied by using a permanent magnet, illustrated in Fig. 4a.
If you are using a magnet sheet, you can cut magnets out to match almost any size and shape.
The culicoids were collected during October 14 16 by using a Mosquito Magnet Independence trap (Mosquito Magnet, Lititz, PA, USA) baited with carbon dioxide and octenol.
CD24−/CD44+/ESA+ cells were then enriched by using a MACS magnet and MS columns (Miltenyi Biotec).
If you're not sure where the two poles are located on the magnet, test them using a second magnet.
Then magnetic beads were removed using a DynaMagTM-2 Magnet (Life Technologies).
The probe:rRNA hybrids were then captured by magnetic beads and removed using a magnet.
The magnetic and nonmagnetic particles were separated by using a magnet.
That is, until you start using a magnet.
Labelled cells were then removed using a magnet.
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CEO of Professional Science Editing for Scientists @ prosciediting.com