Sentence examples for using a QIAquick mini from inspiring English sources

Following second strand amplification, 3.1 μg of purified cDNA was obtained using a QIAquick Mini Elute kit (Mississauga, On, Canada).

Finally, the DNA was purified using a QiaQuick PCR purification kit (Qiagen).

The cDNA fragments were purified using a QIAquick PCR extraction kit.

For use as quantitative standards, the products of the Mycoplasma FRET-PCR on vaginal swabs were gel purified using a QIAquick Gel Extraction Kit (Qiagen, Valencia, CA, USA).

The amplified library was purified using a QIAquick PCR Purification Kit, quantified on the Agilent 2100 Bioanalyzer and finally sequenced on an Illumina Hiseq 2000 instrument.

DNA fragments between 400 600 bp were then selected on a 2% agarose gel and purified using a QIAquick Gel Extraction Kit.

PCR products were analyzed on agarose gel, purified using a Qiaquick PCR purification kit (Qiagen, Valencia, CA, USA), and quantitated prior to DNA sequencing.

The PCR conditions were as described elsewhere (Miyahara et al. 2013), and amplicons were purified using a QIAquick PCR Purification Kit (Qiagen K. K., Tokyo, Japan).

The expected size band (680 bp) was excised from the gel and eluted using a Qiaquick Gel Extraction kit from Qiagen.

Samples were purified using a QIAquick gel extraction kit.

Products of the secondary PCR reaction were purified using a QIAquick purification column (QIAGEN).