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The individual shRNA expression plasmids used as PCR templates were constructed as part of another project using a Phi29 template extension method as previously described [17].
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Davies et al. 2003 [32] built homology models of DRB1*0301, DRB1*0401, and DRB1*1101 using a DRB1*0101 template structure and docked peptides into these MHC models using simulated annealing optimization with the AMBER force field in explicit water.
This was performed using an SPM8 template for [F]FDG-PET.
We routinely use a 3D templating system in the preoperative planning to select the appropriate implant design and size.
DNA synthesis assays were performed as described [38] using a 75mer oligonucleotide template containing a G or 8-oxoG residue at the 45th position annealed to a 44mer oligonucleotide primer [25].
The pBS32-CAG-H2B-citrine was constructed analogously using a H2B-citrine template kindly provided by Michael Elowitz (Caltech).
The samples were transported back and later examined in the Njala University laboratory using the Kato-Katz method using a 41.7 um template (Vestergard Asia, PVT Ltd ,India).
Relative values were calculated by comparing the average C t values from each sample against a standard curve generated using a TSR8 control template.
A 3-D hierarchical nanostructure was synthesized using a 2-D nanosheet template, as shown in Figure 6.
Reactions were carried out in triplicates according to manufacturer instructions using a 20 ng cDNA template for each reaction.
Using a 9" plate as a template, make circles with the dough.
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