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Fluorescence readings were performed in 96-well microplates, using a PHERAstarPlus machine.
100 μl of Caspase-Glo reagent were then mixed into each well an incubated for 1 h at room temperature, before reading luminescence intensity using a PHERAstarPlus machine (BMG Labtech, Aylesbury, UK).
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HBR: Without using a time machine.
I sleep using a breathing machine.
Analysis was performed using a BD LSRII machine.
X-ray images were acquired using a microradiography machine (Faxitron Corporation).
Ligations were performed overnight using a PCR machine.
Gently agitate the SDS solution using a shaking machine.
Haemoglobin level was determined using a haemocue machine.
Real-time PCR was performed using a Quantica machine (Techne).
Using a drilling machine.
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