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Mycoplasma contamination was checked using a Mycoalert kit (Lonza Rockland Inc., ME, USA).
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To monitor the possibility of mycoplasma infection in the mouse skin tissues, the levels of mycoplasma pathogens were detected in a variety of tissue lysate (e.g., whole cell lysate, nuclear extract) using a MycoAlert Mycoplasma Detection Kit purchased from Lonza (Rockland, ME), and the results were negative.
Mycoplasma testing was carried out routinely using a Mycoalert mycoplasma detection kit (Lonza, Rockland, USA) and following the manufacturer's instructions.
Spent media were routinely tested for mycoplasma using the MycoAlert Kit (Lonza, Basel, Switzerland).
All cells were free of mycoplasma, as determined by Characterized Cell Line Core Facility at M. D. Anderson using the MycoAlert kit (Lonza, Basel, Switzerland).
Mycoplasma-positive PCR test results were confirmed using a detection kit (MycoAlert®; Lonza Rockland, ME) based on enzymatic ATP-conversion combined with luminescence measurement.
Consider using a bandolier kit.
RNA preparation was undertaken using a commercial extraction kit.
Phosphatase activity was assessed using a commercially available kit.
These variables are measured using a portable field kit.
Extracellular DNA was measured using a Picogreen dsDNA kit.
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