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Plasmid DNA was extracted by using a Miniprep Kit (Qiagen).
The plasmid DNA mixture was extracted from the cell pellets using a miniprep kit.
High quality plasmid was first prepared from 200 ml overnight culture of cells by standard alkaline lysis and phenol-chloroform extraction protocols, then further purified using a miniprep Kit (Qiagen, Valencia, USA).
Plasmid DNA was extracted from E. coli DH5α strains using a miniprep kit (Qiagen).
Plasmid DNAs were purified using a miniPrep kit (Qiagen, Valencia, CA).
Single bacterial colonies picked from selective agar plates were grown in 5 ml LB, after which the plasmid DNA was extracted and purified using a miniprep kit (Qiagen).
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Highly purified plasmid DNAs containing ILK, ILKK220A, or α-parvin were then prepared using a miniprep DNA purification kit (QIAGEN).
Plasmids were purified using a miniprep plasmid extraction kit (Qiagen).
For the several isolates, existence of plasmid was confirmed to isolate plasmid DNA using a Miniprep DNA purification kit (Takara).
Plasmid, pUCP-pS-pcrV was purified using a Qiagen miniprep kit.
Following incubation at 37°C, cells were washed from the plates with L-broth, and plasmid DNA was purified from the cells using a Qiagen miniprep kit.
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