Sentence examples for using a Mastercycle gradient from inspiring English sources

All DNA amplifications were carried out using a Mastercycle gradient (Eppendorf) and standard PCR protocols.

Microsatellites were amplified with a Mastercycle Gradient machine (Eppendorf, Hamburg, Germany) using the Qiagen® Multiplex PCR Kit (Qiagen, Hilden, Germany).

Cyt b sequences were amplified with a Mastercycle Gradient machine (Eppendorf, Hamburg, Germany) using a Maxima Hot Start PCR Master Mix (Fermentas GmbH, St. Leon-Rot, Germany) and zcyt-1F (5′-taatggcaagcctccgaaa-3′) and zcyt-2R (5′-ctgagctactaatgcaggatca-3′) as forward and reverse primers, respectively.

Finally, the system was relaxed using a conjugate gradient algorithm.

Finally, the minimization was done using a conjugate gradient algorithm.

Scattering effects are modelled using a local gradient shading model.

Each gradient was fractionated into 40 fractions using a Density Gradient Fractionation System (Brandel, Gaithershurg, MD).

Interaction chromatography is generally performed using a solvent gradient or a temperature gradient.

The gradients were eluted using a Brandel Gradient Fractionator and prepared for qMS and EM analysis.

SDS-PAGE was carried out using a 5 20% gradient gel.

Mitochondria and MAM were purified using a sucrose gradient.