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The resulting cDNA was purified using a MINElute Kit (Qiagen).
Products >70 bp long were purified by using a MinElute kit (QIAGEN, Valencia, CA, USA).
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The amine-modified DNA was purified using a MinElute PCR Purification Kit (QIAGEN), following the instructions in the kit, except that the washes were performed twice instead of once and the probe was eluted with 0.1 M NaHCO3.
Linearized vector was excised from the gel and the DNA extracted using a MinElute Gel Extraction Kit (Qiagen).
For 1 kb and 2 kb fragments, 1 and 2 kb bands were extracted from a partial digestion of genomic DNA resolved on a 1% standard agarose (Sigma-Aldrich, St . Louis MO) using a MinElute Gel Extraction Kit (Qiagen).
In the last step of the protocol, the indexing amplification was run into plateau (20 cycles) and the reactions were purified using a MinElute PCR purification kit (Qiagen, Hilden, Germany).
Fifty ng of CIP-treated template was incubated with 20 U TdT (NEB) for 20 minutes at 37°C, and the reaction product was isolated using a MinElute Reaction Cleanup Kit (Qiagen).
Amplification was carried out for 40 cycles at 94°C for 30 sec, 50°C for 30 sec and 72°C for 4 min, and one cycle at 72°C for 10 min. PCR products were visualized on a 1.5% agarose gel and DNA was recovered by using a MinElute Gel Extraction kit (Qiagen) as specified by the manufacturer.
PCR products were purified using a MinElute PCR Purification Kit (Qiagen, Hilden, Germany).
The cDNA was purified using a MinElute PCR Purification Kit (Qiagen).
DNA 300 bp to 700 bp was isolated using a MinElute Gel Extraction Kit (Qiagen).
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