Sentence examples for using a MACSQuant analyzer from inspiring English sources

Samples were analyzed using a MACSQuant Analyzer and the MACSQuantify software (both Miltenyi Biotec).

The DNA content of cells was analyzed using a MACSQuant analyzer.

The cells were washed, incubated with PE-labeled anti-human IgG Fc (HP6017) (Biolegend) or corresponding isotype-control antibodies (Affymetrix, Santa Clara, CA, USA) in buffer for 30 min at 4 °C, washed, re-suspended and analyzed using a MACSQuant Analyzer (Miltenyi Biotech K.K .. Before using the analyzer, 4 μg/ml PI (Sigma-Aldrich) was added to the sample to exclude dead cells.

To assess HER2 expression levels, cells were incubated either with phycoerythrin (PE -labeled anti-human HER2 (24D2) or with corresPE -labeledtype-control antibodies (BioLegend, San Diego, CA, USA) in buffer (1 % FBS, 2 mM EDTA and 0.1 % NaN3 in PBS) for 30 min at 4 °C, after wHER2 they were washed and then analyzed using a MACSQuant Analyzer (Miltenyi Biorech K.K., Bergisch Gladbach, Germany).

Experiments were performed using a MACSquant Analyzer (Miltenyi Biotech, CA) and analyzed using the FloJo software package (vers. 9, Tree Star Inc., OR).

Cells were washed with FACS buffer (50 mM sodium citrate), treated with RNase, stained with propidium iodide (4 μg/ml final), and analyzed by using a MACSQuant analyzer (Miltenyi Biotech).

Stained cells were analysed using a MACSQuant analyser (Miltenyi Biotec, Germany).

Finally, samples (60,000 cells) were analyzed using a MACSQuant (Miltenyi) flow cytometer, with a 488 nm laser to excite SYTOX Green and a bandpass filter 500 550 nm to detect fluorescence.

ROS accumulation was analyzed using a MACSQuant® (Miltenyi Biotec) flow cytometer and FlowJo software.

Cells were washed in binding buffer and analyzed using a MACSQuant flow cytometer with MACSQuantify software (Miltenyi Biotec).

Following washing, cells were analyzed on a FACScalibur using CellQuest-Pro (BD Biosciences, Oxford, UK), or on a MACSQuant Analyzer (Miltenyi Biotec).