Sentence examples for using a Limk1 cdna from inspiring English sources

A 5′-Hprt clone was obtained by screening the mouse 5′-Hprt129/SvEvBrd-derived 5′-Hprt129/SvEvBrd-derived 5′-Hprt129/SvEvBrd-derived

To this purpose, we cloned amplified chicken p53 using a DT40 cDNA library made by the reverse transcription reaction.

FISH was performed as previously described (Pinkel et al, 1988) using a COL7A1 cDNA probe labelled with Spectrum Red as described in the Supplementary Materials and Methods.

Differential expression between hormone-refractory CWR22R prostate cancer xenografts and xenografts of the parental hormone-sensitive CWR22 strain was examined using a 588 cDNA Clontech filter microarray.

We profiled gene expression in earthworms exposed to TNT (50 mg/kg soil), RDX (30 mg/kg soil) or a mixture of these two explosives using a 4K cDNA microarray.

C-terminally FLAG tagged RNF20 was PCR cloned between the KpnI and EcoRV sites of pcDNA3.0 (Invitrogen) using an RNF20 cDNA as template (Open Biosystems, MHS4426-98361130).

This transgenic line, named Fsmad1, was made with the same procedure and construct used to generate the COET line, except that we used a SMAD1 cDNA instead of the Tbx1 cDNA.

The 35S PEX5 construct used a PEX5 cDNA [ 17 ]; for 35S PEX5 genotyping, PEX5 primers spanning introns were used to amplify larger genomic PEX5 or the smaller PEX5 cDNA products.

Total RNA was extracted using an RNA extraction solution (BioAssay Co., Daejeon, Korea) and reverse transcribed to cDNA using a 1st Strand cDNA synthesis kit (BioAssay Co ., according to the manufacturer's protocol.

The cDNA was synthesized using a 1st-STRAND cDNA Synthesis Kit (Fermentas, Pittsburgh PA, USA).

Microarray analysis was performed using a 21.8K spruce cDNA microarray.