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The sizes of the nanocrystals were determined by DLS measurements using a HeNe source (λ = 632.8 nm) and a photomultiplier as detector.
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The Raman spectra were acquired using a HeNe laser (λ = 633 nm) as radiation source.
Excitation of Toto-3 was achieved using a HeNe 633 nm laser and emissions were collected using a 680±30 nm filter.
Tissue immunolabeled with Cy5 was excited using a HeNe laser (633 nm) and the resulting images collected in the emission range 640 740 nm.
The sample was illuminated at normal incidence on its center using a HeNe laser (632.8 nm), and a detector placed on a rotating arm was able to perform a 360° measurement of the scattering intensity.
As guiding light, a HeNe source (633 nm) emitted continuously at 1.2 mW.
We used a HeNe laser (JDSU, USA) with 632.8 nm and a maximum output power of 35 mW as the light source.
DOPE-Rhod was excited at 543 nm using a green HeNe laser and HPTS was excited using the 488 nm Argon laser line.
Moreover, we show that also off-resonance measurements, using a standard HeNe laser at 633 nm, allow for the SERS characterization of the ligand shell of NPs.
All of the FPI optics are calibrated by using a frequency-stabilized HeNe laser (Spectra Physics 117A-LW) at a wavelength of 632.8 nm.
Dynamic light scattering was measured with NanoBrook 90Plus Zeta Particle Size Analyzer with excitation at 640 nm using a 40 mW HeNe laser (Brookhaven Instruments Corporation).
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