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Briefly, cells were lysed (using a French pressure cell) in lysis buffer containing 50 mM Tris pH 8, 300 mM NaCl, 2 mM TCEP, and Complete protease inhibitor cocktail tablets (Roche Applied Science, Indianapolis, IN).
The cells were lysed using a French pressure cell.
Cells were broken using a French pressure cell (15.2 kPa) and centrifuged (5200 × g, 30 min).
The cells were lysed using a French pressure cell and clarified by centrifugation.
Cells were harvested by centrifugation and lysed using a French pressure cell.
Cultures were induced for 4 6 hr, harvested by centrifugation and lysed using a French Pressure Cell.
Similar(36)
The resuspended cells were lysed using a French press, at a pressure of 1500 psi.
Inside out membrane vesicles were prepared by cell lysis using a French Press at a pressure of 10000 psi (1 psi=6.9 kPa), followed by low- and high-speed centrifugation of the cell lysis mixture [ 15], and were used directly for flippase measurements.
Bacteria from early-stationary phase (8 h) cultures were harvested by centrifugation (8,000× g for 10 min), washed with deionized water, and disrupted by high pressure (18,000 psi) using a French Press.
Cells were lysed by using a French Press.
Cells were lysed using a French press at 1000 psi.
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