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Analysis was performed using a Fortessa analyzer (Beckman Coulter, High Wycombe, UK) and data analyzed using FlowJo v7.6.5 (Ashland, OR).
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Cell-cycle profiles were obtained using a Fortessa cell analyzer (BD Biosciences) and produced using the FlowJo software (Tree Star).
Frequencies of infected cells and lymphocyte subsets in PBMC were determined by flow cytometry using a Fortessa Cell Analyser (BD Biosciences).
Flow cytometry was performed using a Fortessa (BD Biosciences) and analyzing data with FlowJo software.
GFP and RFP expression was assessed using a Fortessa Flow cytometer.
For flow cytometry, single cell suspensions were analysed using a Fortessa flow cytometer and analysed using FlowJo (Tree Star).
GFP (Green Fluorescent Protein) and RFP (Red Fluorescent Protein) was assessed using a Fortessa flow cytometer (BD Biosciences, Oxford, UK).
The concentrations of PG were measured bedside by the glucose oxidase method using a glucose analyzer (YSI model 2300 STAT plus analyzer; Yellow Springs Instrument Inc., Yellow Springs, OH, USA).
The radio-isotope signal was detected using a bioimaging analyzer BAS-2500 (Fujifilm).
Serum aspartate transaminase (AST) and alanine transaminase (ALT) were measured using a biochemical analyzer (AU480, Beckman Coulter, CA, USA).
Thermogravimetric analysis was carried out using a Q5000IR analyzer (TA instruments) with an automated vertical overhead thermobalance.
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