Sentence examples for using a FACScaliber machine from inspiring English sources

Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine (BD).

Flow cytometry was performed immediately or within 24 h of permeabilization using a FACScaliber machine. 1 × 105 gated events were recorded.

Viability was determined by propidium iodide [16] exclusion and flow cytometry using a FACScaliber (BD Pharmingen).

Cells were analyzed using a FACSCaliber (BD Biosciences) flow cytometer (Beckton-Dickinson).

Flow cytometry parameters were measured using a FACSCaliber instrument (BD Biosciences) and analysed with CellQuestPro (BD Biosciences).

Stained cells were briefly sonicated as described above and DNA content was measured using a FacsCaliber flow cytometer (BD Biosciences, Woburn, MA) at a maximum flow rate of 500 cells/s.

HeLa cells (1×106 cells) were incubated with 0.5 ml of propidium iodide/RNase buffer (BD Bioscience) after fixation with 70% ethanol for 1 hour at -20°C, and DNA content was measured using a FACSCaliber (Becton-Dickinson).

Surface receptor immunoreactivity was assayed by incubating intact, non-permeabilized cells with Alexa647-conjugated anti-FLAG M1 antibody (1 µg/ml for 45 min), washed, and analyzed by fluorescence flow cytometry using a FACSCaliber instrument (Becton Dickenson).

All the data was generated using a FACSCaliber flow cytometer.

Cell cycle analysis was performed by using a FACSCaliber (Becton Dickinson, San Jose, CA).

Specimens were analyzed using a FACScaliber cytometer (BD Biosciences, San Jose, CA) (10,000 events) in the FL1 channel (533 ± 30 nm).