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Genomic DNA was extracted from plant leaf material using a DNeasy Mini Kit (Qiagen).
DNA was extracted from 250 μl of supernatant from five pooled swabs (2 pools/flock) and from tissue homogenates using a DNeasy Mini Kit (QIAGEN, Germany) according to the manufacturer's instructions.
Genomic DNA was isolated from purified Teff and, separately, Treg cells, from each subject's samples using a DNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions and controlling for cell number.
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Genomic DNA was extracted from leaves using a DNeasy plant mini kit (Qiagen).
Genomic DNA was extracted from silica-gel-dried leaf tissue using a DNeasy plant mini kit (Qiagen) according to the manufacturer's protocol.
Total genomic DNA was isolated from leaves of a single plant from each line using a DNeasy plant mini kit (Qiagen).
Total genomic DNA in BC1F1 plants was extracted from 200 mg of fresh leaf tissue using a DNeasy plant mini kit (Qiagen, Valencia, CA, USA).
Genomic DNA was isolated from 10-day-old B. juncea, B. nigra and B. campestris seedlings using a DNeasy plant mini kit (Qiagen, Valencia, CA).
Genomic DNA isolated from roots of healthiest transgenic roots that displaying the strongest eGFP fluorescence and control soybean plants using a DNeasy plant mini kit (Qiagen, USA).
DNA was isolated using a DNeasy plant mini kit (Qiagen), and 5-50 ng of DNA were used in PCR with Ex taq (Takara) with gene specific primers (Additional File 1) under the following conditions: 95°C for 5 minutes, followed by 40 cycles of 95°C for 30 seconds, 59°C for 30 seconds, and at 72°C for 1 minute per 1 kbp of predicted product size.
For tag detection from nodules, detached nodules were washed twice with a solution of 0.5%-SDS-100 mM NaCl and then soaked in 70% ethanol for 5 min. After five times washes with sterilized water, total DNA was isolated using a DNeasy plant mini kit (Qiagen, Hilden, Germany).
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