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Genomic DNA was extracted using a DNeasy extraction kit (Qiagen).
DNA was extracted using a DNeasy extraction kit (Qiagen, Valencia, CA).
DNA was extracted from blood, feathers, and organ tissues using a DNeasy Extraction Kit (QIAGEN, Inc ., with 30 µl of 100 ng/ml dithiothreitol (DTT) added to the extraction buffer when working with feathers.
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DNA was extracted using a DNeasy tissue extraction kit (Qiagen, Germany) and 5 μl of template DNA (50 ng/μl) was used in simplex PCR to detect FIA, FIB, FII, I1 and N groups.
Genomic DNA was extracted from muscle or fin clips using a DNeasy Tissue Extraction Kit (Qiagen, Valencia, CA).
DNA was extracted from both blood and feather tissues using a DNeasy Tissue Extraction Kit (QIAGEN Inc., Valencia, CA).
Total genomic DNA was extracted from blood or from toe-pad tissue for museum specimens using a DNeasy Tissue Extraction Kit (QIAGEN Inc).
Total genomic DNA was extracted from the tissues using a DNeasy tissue DNA extraction kit (Promega) following the manufacturer's instructions, and was dissolved in TE buffer.
DNA was extracted using a DNeasy blood and tissue extraction kit (Qiagen).
Genomic DNA extraction was isolated from fresh peripheral blood samples using a DNeasy blood and tissue extraction kit according to the manufacturer's protocol (Qiagen, Hilden, Germany) and quantified using a spectrophotometry (Biophotometer Plus, Eppendorf, Germany).
Briefly, we extracted genomic DNA from 115 F2 hybrid individuals using a DNeasy Blood and Tissue extraction kit (Qiagen, Valencia, CA, USA).
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