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RNA was DNase-treated using a DNAfree kit (Ambion).
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We quantified RNA preparations spectrophotometerically, removed residual DNA using an Ambion DNAfree kit, and generated cDNAs using an Ambion Retroscript kit.
The samples were DNAse treated using the DNAfree kit (Ambion) followed by an additional ethanol precipitation.
RNA samples were DNAse treated using the DNAfree kit (Ambion, Carlsbad, CA) followed by an additional ethanol precipitation.
Contaminating DNA was removed using the DNAfree kit [Ambion].
DNase digest was performed using the DNAfree Kit (Ambion).
RNA was treated with DNase using DNAfree kit (Ambion, Cambridgeshire) and 1 μg was used for RT-PCR using Retroscript kit (Ambion, Cambridgeshire, UK).
RNA was treated with DNase using DNAfree kit (Ambion, Cambridgeshire, UK) and 1 μg was used for RT PCR using Retroscript kit (Ambion).
Bacterial cells pelleted by centrifugation were lysed mechanically with 0.25 0.5 mm glass beads (Sigma) in Tissue Lyser (Qiagen, Hilden, Germany) for 6 min at 30 Hz. RNA was purified by using the RNeasy Mini kit (Qiagen), then treated with DNase using DNAfree kit (Ambion, Cambridgeshire, UK) and checked for DNA contamination by PCR amplification without prior reverse transcription.
Consider using a bandolier kit.
RNA preparation was undertaken using a commercial extraction kit.
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