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Briefly, Sailfish uses transcript sequences to build a kmer index over all annotated transcript sequences.
The ASD pipeline uses transcript (EST/mRNA) sequences to decipher splice patterns.
As shown in Figure 1A, Alt Event Finder uses transcript isoform annotation from Cufflinks (GTF format) or Scripture (BED format) as input.
It is also worth noting that the variant filtering approach implemented in OVA uses transcript, rather than gene, sequences in order to take codon position plurality (Subramanian, 2015) into account.
GLEAN uses transcript alignments to support splice predictions, but does not merge or split genes based on transcript alignments, while MAKER2 creates gene models that best agree with transcript alignments, and works best with highly reliable transcript assemblies.
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This addresses a major gap in much classroom discourse analysis, which typically uses transcripts of classroom talk, although only a minority of students' voices are recorded in a transcript.
As a default, JAFFA uses transcripts from GENCODE [ 27] as a reference.
The accuracy and technical reproducibility of the method suggests that expression profiling using transcript amplification and high density oligonucleotide microarrays can be used on a routine basis.
ITS pseudogenes were identified experimentally using transcript analysis [17].
sept9 isoforms and ef1α were amplified using transcript specific primers.
We extended the available gene prediction using transcript sequence evidence.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com