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Therefore, we used the wild type strain, Y-94, in our study.
L-Galactonic acid production was not observed in either process using the wild type strain.
Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation).
Site-directed mutagenesis using the Stratagene Quick-Change II kit (Stratagene) was used to generate the P58S mutant using the wild type cDNA.
The experiments described below were to further dissect this pattern of motility, using the wild type strain B10 and mutant derivatives.
The concentration of Mn2+ for strain exposure was determined experimentally using the wild type parental strain, BY4741.
We used the wild type yeast BY4741 strain as a standard for the real-time PCR.
Towards this end we used the wild type coding sequence from A. alpina Pajares and pep1-1.
The haplotype construction used the wild type (Wt) or the variant for each allele at rs12998 and rs5780218 polymorphisms.
The relative MPV17 expression levels for homozygous and heterozygous dogs were calculated using the wild type sample as a calibrator.
For donor strain we used S. Typhimurium FSL R8-3980, whash has a chromosomally inserted cat gene (chloramphenicol resistance); as recipient we used the wild type strain FSL R8-3981.
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