Sentence examples for uses the sanger method from inspiring English sources

Identification was accomplished with tools and techniques that are accessible to routine laboratories, including a low-cost "in-house" DNA extraction protocol and an automatic sequencer that uses the Sanger method.

The PCR products were also sequenced using the Sanger method for validation.

The entire coding sequence of MYOC was amplified and sequenced using the Sanger method.

The E. coli DH10B strain was used as the cloning host, and six clones were selected to be sequenced using the Sanger method at MACROGEN (Seoul, South Korea).

PCR products of the bacterial DNA were sequenced using the Sanger method of sequencing with 3500 ABI genetic analyser, at Inqaba Biotechnical Industries, South Africa.

PCR products were sequenced using the Sanger method.

The p.Q485H mutation identified via WES was confirmed using the Sanger method.

Each sequence generated using the Sanger method had a perfect match to overlapping fragments.

Sequences reads generated using the Sanger method and by the 454 pyrosequencing were combined and assembled using a hybrid assembly approach developed at the JCVI [18].

PCR primers were designed to target the region surrounding the polymorphisms and the products from each individual were sequenced using the Sanger method.

The RT-PCR protocol for sequencing using the Sanger method and pyrosequencing [48] are presented in the SI, as well as the phylogenetic analysis.