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Dr. Weinstein, a licensed recreational pilot, uses the same light touch when he is at the controls of his twin-engine Cessna.
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However, samples measured 24 hours post PDT using the same light dose showed less CC3+ fragmented nuclei cells since those that had previously undergone apoptosis become detached and were not being detected at this time point by the assay.
All images were segmented using the same light threshold, mask smoothing and object size filters.
Here, using the same light source as that used for behavioural testing, we found a clear, melanopsin-driven c-Fos induction in medial visual/retrosplenial cortex (Figure S1).
Briefly, all images from a given experiment were acquired in a single session using the same light intensity and filter settings with the white balance of all images standardised.
We used the same light source and polarizer reported in [12] and the negative refractive transmitted light was captured by a CCD camera (KP-D50, Hitachi)) coupled with a singlet lens (focal length of 8 mm, NT-45114, Edmund Optics) placed at 8 mm from the sample.
High light treatments (500 and 1000 μmol photons m-2 s-1) were given using the same light source and were started immediately after the diurnal dark period.
In other ongoing studies using Advillin-cre/ChR2 and trkB-CreER-ChR2 mice we can evoke action potentials in LTMRs directly using the same light stimulus.
The rinsed samples were then postcured for 10 min using the same light source and intensity without the photomask and under ambient conditions to maximize the monomer conversion.
Plants on plates and trays were grown in growth chambers using the same light and temperature conditions as described earlier by [ 30].
However, in ongoing studies using Advillin-cre- and trkB-CreER-ChR2 mice, action potentials can be evoked in Aβ and Aδ LTMRs using the same light stimulus (not shown).
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