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This test uses the Affymetrix microarray platform and screens for 27 different alleles of the CYP2D6 gene (including gene duplications and deletions) and 3 alleles of the CYP2C19 gene.
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Data were analyzed using the Affymetrix Microarray Suite 5.0.
All GEPs were performed using the Affymetrix microarray platform and standard procedures.
Gene Chips were scanned with an argon-ion laser and data visualized using the Affymetrix Microarray Suite 5.0 software.
To facilitate comparisons among microarrays, global scaling was performed using the Affymetrix Microarray software (MAS 5.0) and the target intensity was set to 500.
In each cell line, we compared the expressions of 22.000 genes in vitro and in the xenografts, using the Affymetrix microarray approach.
The GeneChips data analysis was performed using the Affymetrix Microarray suite 5 and the GeneSpring 5 (Agilent Technologies, Palo Alto, CA) analysis package.
This analysis resulted in identification of 4,120 expressed genes and 2,682 unexpressed genes in HeLa cells, based on the absent and present calls using the Affymetrix Microarray Suite®, version 5.0 [24].
To elucidate the molecular mechanism of the synergistic lethality between ABT-869 and SAHA, we compared the gene expression profiles of MV4-11 and MOLM-14 cells treated with DMSO control, ABT-869, SAHA and combination therapy using the Affymetrix microarray platform.
Annulus gene expression analysis used the Affymetrix microarray system.
These experiments all used the Affymetrix microarray platform.
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