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To assign this element to a specific clade, an automated tool (RtClass1, http://www.girinst.org/RTphylogeny/RTclass1/) [ 24] was employed that uses phylogenetic analysis of the RT domain protein.
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In addition to using phylogenetic analysis of nucleotide sequences, it would be useful to include microsatellite loci or single nucleotide polymorphisms (Miller et al. 2007; Hohenlohe et al. 2010).
Lanyon (1994), Lanyon and Omland (1999), and Barker et al. (2008) used phylogenetic analysis of mitochondrial DNA (cytochrome b) to investigate the relationships within Agelaius blackbirds.
Here we describe a tool (RTclass1) designed for the fast and accurate automated assignment of novel non-LTR retrotransposons to known or novel clades using phylogenetic analysis of the RT domain protein sequences.
They used phylogenetic analysis of catalytic domains to build classification trees, used as a basis for the classification in 55 distinct subfamilies.
Additionally, we used phylogenetic analysis of partial β-tubulin gene sequences to confirm that the fungal isolates studied here represent a single species.
Under the most parsimonious scenario, the Plantae share a unique common branch that defines the point of entry of the primary endosymbiont [1], [5], [6], [7], although the monophyly of this group remains to be unambiguously demonstrated using phylogenetic analysis of nuclear genes [8], [9].
We attempted to corroborate this result using phylogenetic analysis of Tc1/ mariner transposase amino acid sequences.
Species boundaries were first established a posteriori using phylogenetic analysis of 246 Haemophilus genomes (Fig. 1).
Using phylogenetic analysis of the complete S, it clustered as an outlier of subgenotype D4, whereas following complete genome phylogenetic analysis it clustered with D6.
Putative species were inferred based on 16S rRNA phylogenies (using 16S rRNA sequences extracted from the draft genome sequences) and confirmed using phylogenetic analysis of 31 protein sequences.
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