In some respects, there are many similarities to the development of penicillin, where the first reported US patient treated for streptococcal septicemia used one half of the total available supply produced by Merck & Co (White-house Station, NJ).
The leaf tissue mRNA samples were sequenced on the 454 Titanium FLX platform and each sample used one half picotiter plate.
I've lost count of the times I've explained that I'll use one half for lunch in a soup, and the other half in dinner, or double the quantities of the recipe and freeze the leftovers.
While the information is open to any code, information security is guaranteed by using one half of a digital signature (Elliptic Curve Digital Signature Algorithm).
We randomly partitioned the data set and used one half for training the classifier and the other half for testing.
Early DNA extractions were performed using one half of each individual; subsequently, only two appendages (one gnathopod and one pereiopod) from each individual were used for DNA extraction.
Thraustotheca clavata DNA was sequenced using one half plate of 3 kb paired-end Roche 454 FLX and two lanes of 108 cycle paired-end Illumina.
Using one half of a 454 run, we were able to barcode PCR amplicons from 1710 samples of rodents, corresponding to 24 species.
Pools A and B were simultaneously processed in one GS FLX run using one half of a 70×7575 mm Pico-Titer plate device (Roche Diagnostics GmbH) and one GS LR-70 sequencing kit (Roche Diagnostics GmbH).
Achlya hypogyna DNA was sequenced using one half plate of Roche 454 FLX (Margulies et al. 2005) and one lane of 72 cycle paired-end Illumina-Solexa (Bentley et al. 2008).
At the end of the experiments, 29 or 30 days following the start of the treatment with inhibitors, rats were killed and the right kidneys were sagittally sectioned to use one half fixed in formalin for immunohistochemistry for localization CGL and CBS proteins in the renal tissues, and the other half was snap-frozen for eventual western blot analysis.
