Mr. Jagger never had much use for this method.
Different thresholds are used for this method.
Two different importance sampling distributions can be used for this method.
The low density mosquito number used for this method made it easy observation for the mosquito behaviour.
Techniques used for this method are solely based on statistics, e.g., Weibull analysis and log-normal and Poisson laws [34].
Since any kind of features can be used for this method, we reuse the color histograms that were used in the above process for saving the computations.
Different to the RT-qPCR technique, the RNA samples used for this method were ligated with 5′-adaptors and 3′-adaptors before the reverse transcription reaction.
The parameters used for this method were samplingFactor = 3, saddleHeight = 2, overlappingFactor = 0.7.
The implementation used for this method is a custom software, which has already been used in [11,12].
A designated reagent system was used for this method, according to the instructions for use of the manufacturer (Sysmex, Kobe, Japan).
