This method uses a single pairs of primers to amplify specifically two products simultaneously, one from a copy-constant reference locus and the other from the copy variable test locus of interest.
Essentially, you can use a single pair of chopsticks from start to finish – beating the eggs, frying the ingredients, tossing the noodles, and eating.
Fifteen selection cycles were considered, using a single pair mating design.
Intraclass correlation coefficients and 95%% confidence intervals (CI) were estimated using a single pair of measures per patient.
Instead of using a single pair of dictionaries as done in [11] and [12], multiple directional dictionaries are proposed as done in [10].
The present procedure using a single pair of primers for two fragments can overcome disadvantages in quantitative analysis using multiplex PCR.
Thus, it was possible to amplify DNA from both Old and New World pathogenic Leishmania complexes using a single pair of primers deriving from the conserved region of the mini-exon tandem repeat.
A novel primer design method is described for site-directed fragment deletion, insertion, and substitution by PCR that is based on inverse PCR using a single pair of partially complementary primers.
Even though it is well known that electromyography (EMG) characteristics are influenced by electrode placement it is common to use a single pair of sensors per muscle for EMG.
