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This program allows analyzing large combined data sets and uses a GTR model based approach with internal estimation of all free model parameters for all data partitions.
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In addition, a Bayesian tree was calculated with MrBayes 3.1 [45] using a GTR model with invariable sites and rate heterogeneity.
The analysis used a GTR model with among site rate heterogeneity (gamma distribution with 8 categories).
RAxML was used to fit 1,000 bootstrapped tree topologies and optimize branch lengths using a GTR model [ 31, 32].
Each search used a GTR model of nucleotide substitution with the gamma model of rate heterogeneity initiated from a complete random starting tree.
PhyML [ 19] was used for the generation of phylogenetic trees using a GTR model estimated gamma distribution and 4 substitution rate categories.
We used a GTR model of substitution with heterogeneity among sites and we allow for different rates among cytb, cox1, cox3 and the non-coding regions.
A phylogenetic analysis using a GTR model in PHYML, established that 13 out of the 15 sequences containing the D67N/K219Q formed a distinct out-group with a bootstrap value of 100%.
Bayesian phylogenetic inference was carried out using a GTR model with gamma distribution and 8 rate categories per partition (all parameters unlinked) and rate multipliers to accommodate rate differences among partitions.
For 28S we used a GTR+G model; for COI we used a GTR model with separate rates for each codon position.
Cox1 uncorrected distances between collection sites ranged from a minimum of 0.5% between those located close to each other (viz., 18 and 28, only 3.5 km apart) to a maximum of 8.9% (corrected to 9.8% using a GTR model) between populations from the two islands (viz., localities 4 and 20, separated by 68 km) or between some Mallorcan populations.
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