Next, the DNA from the bands was sequenced using a DNA sequencer (Applied Biosystems, Foster City, CA, USA).
The PCR products were sequenced using a DNA sequencer (ABI PRISM 3730, Foster City, CA, USA), and the methylation levels were measured according to our previously reported method [ 19].
In order to validate the credibility of the identified eSNP, nucleotide sequences of PCR products containing the eSNP regions were determined by direct sequencing using a DNA sequencer ABI-37300 xl, Applied Biosystems, Foster City, CA, USA).
All T7 DNA was sequenced per Novagen's suggested protocol using a DNA sequencer (Applied Biosystems, 3730XL DNA Analyzer, UM DNA Core).
The hospital then prepares samples and processes them using a DNA sequencer.
Multiplexing using a DNA sequencer has been developed for high-throughput glycoprofiling of APTS-labeled glycans [ 37, 38, 77].
These improvements could include the use of fluorescently labelled primers and fragment analysis using a DNA sequencer to accurately assign repeat sizes.
The PCR products of selective amplifications were separated by capillary electrophoresis using a DNA sequencer (ABI PRISM® 3130 XL Genetic Analyzer, Applied Biosystems, USA).
Here we describe procedures for the conversion of fluorescent differential display results into quantitative transcription profiles, using a DNA sequencer for cDNA fragment separation and DNA fingerprinting software for band matching, data normalization and densitometry.
