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DNA was obtained using Nucleospin Tissue kit (Macherey-Nagel, 740952.5) as described in the user protocol.
Following the GeneJuice transfection reagent (Merck-Millipore UK, Merck KGaA, Darmstadt, Germany) user protocol, cells were transfected with 1 µg of the appropriate DNA.
The two flanking fragments were introduced into pRFHU2 following the USER protocol described by [ 87] and the resulting plasmid was introduced into E. coli DH5α chemical competent cells.
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Thus, the biosensor system can allow users to perform rapid whole blood diagnostics with minimal user protocols, in 5 min.
The level of producing nitric oxide (NO) by ASC and ELC was measured by colorimetric (Nitric Oxide Assay Kit, Abcam) according to the user protocols.
cDNAs were then modified, concentrated, and hybridized to the array as recommended in the Genisphere users' protocol.
The Uu and Un protocol stacks are developed in accordance with the LTE-A protocols and end-user protocols.
There are two widely accepted protocols for video streaming, RTP over User Data Protocol (UDP) and Hyper Text Transport Protocol (HTTP) over Transmission Control Protocol (TCP).
A VoIP data frame consists of the user datagram protocol (UDP), internet protocol (IP) and MAC layer headers, and the user payload.
The video content is transmitted using User Datagram Protocol over Real-Time Transport Protocol (RTP).
DTLS is a security solution at the transport layer, used for applications that operate over the User Datagram Protocol (UDP) or Transmission Control Protocol (TCP).
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