This article reports the development of those universal primers for short-range PCR, confirms their usefulness in sequencing the whole chloroplast genome using Liquidambar formosana as an example, and compares the three genomes determined using short-range PCR, long-range PCR, and the NGS methods.
As the HGP got underway, a number of philosophers weighed in on its scientific merit in terms of cost, potential impact on other areas of research, ability to lead to medical cures, and the usefulness of sequence data (Kitcher 1995; Rosenberg 1995; Tauber and Sarkar 1992; Vicedo 1992).
The present study demonstrates the usefulness of sequence-based genome mining for potential drug targets representing one possible route for the prevention of matrix degradation of the mucosal epithelium by H. pylori and other pathogens.
We have proved the usefulness of sequence profiles in loop structure prediction (Oliva et al. 1998; Fernandez-Fuentes et al. 2005).
This finding differed from the biochemical identification and underlined the usefulness of sequencing to precisely identify gram-negative bacilli that assimilate only a few sugars.
Although several studies have demonstrated its potential usefulness for sequencing small and compact microbial genomes, it was unclear how the new technology would perform in large and highly repetitive genomes such as those of wheat or barley.
This is an active area of research and many important topics remain to be investigated, e.g., a better characterization of the genetic architecture of the traits under selection, methods to successfully include biological information into genomic selection procedures or the usefulness of sequence in multi-breed populations.
Actually, we concur with others [ 64, 65] on the usefulness of CYB sequences – including third codon positions – when they are combined with less saturated sequences.
A number of reference genomes for legumes including M. truncatula, Glycine max and Phaseolus vulgaris are completed [ 32– 34], but the genome of pea has not yet been sequenced and this limits the usefulness of deep sequencing approaches for transcriptomic investigations.
Altfeld et al. [48] demonstrated the usefulness of autologous sequences to detect immune responses against Tat and Vpr.
