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This method represents a useful tool for delineating causal variants by bringing together GWAS signals and the rapidly accumulating variant data from next-generation sequencing.
The use of the rigid ligands as molecular rulers in conjunction with double-mutant cycle analysis provides a means of mapping the relative positions of various residues in the ligand-binding site of any ligand-receptor complex, and thus is a useful tool for delineating the architecture of the binding site.
To further determine the role of S6K1 in the combination of mitomycin C and rapamycin-induced apoptosis, PF-4708671, a novel S6K1-specific inhibitor and a useful tool for delineating S6K1-specific roles, was employed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com