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We have created an in vitro model of connector handling with gloves impregnated with a 0.05 McFarland S. aureus solution while instilling saline, lasting long enough to be used as a useful model to test the efficacy of closed needleless connectors.
Generation of PINK1 knockout mice Primary embryonic mouse cultures Table S1 Antibodies SDS-PAGE and immunoblotting Production of stable cell lines Measurement of total and reduced glutathione Live cell imaging ETC activities We first sought to validate the use of the human NSC model, differentiated to human midbrain neurons, as a useful model to test PINK1 function.
A daily connector handling with a 0.05 McFarland S. aureus solution while instilling saline proved to be a useful model to test the efficacy of closed needleless connectors.
This study confirms that the physiological fall in blood lipids in the postpartum period can be a useful model to test the relationship between serum cholesterol levels and mood.
The CF mouse gastrointestinal tract provides a useful model to test potential therapies because of the wide-ranging and dramatic changes that occur compared to control WT mice.
This provides an example of how the APP/PS1 KI mouse could be a useful model to test preclinical therapeutics targeted at reducing oxidative stress.
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We used the embryonic (HEK293), placental (JEG3), and hepatic (HepG2) human cell lines because they are well characterized and validated as useful models to test toxicities of pesticides [ 18– 20], corresponding to what is observed on fresh tissue or primary cells [ 21– 23].
Liver-specific Fxr KO mice fed with alcohol will be a very useful model to further test this hypothesis, because these mice would avoid the problems such as increased bile acids and liver injury in whole body Fxr KO mice.
All students used zebrafish as their model system, as zebrafish are a very useful animal model to test the effects of a drug on a biological response.
In addition, given that our mutant mice have a single base deletion as often observed in patients, they could represent a useful model to set up and test novel gene therapy approaches, such as in vivo gene correction by homologous recombination (38), stem cells, or induced pluripotent stem cells from patients.
We chose KcsA because (i) it is a robust IMP that can be produced in amounts sufficient for extensive in vitro experiments, (ii) its channel activity and thus successful reconstitution can easily be confirmed, and (iii) it has proven useful as a model to test reconstitution protocols.
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