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This Cre-loxP system is widely used in modern molecular biology and is particularly useful in the generation of conditional gene knockouts [20].
This is particularly useful in the generation of dynamic landscapes in spatio-temporal studies as it allows separate ascription of spatial and temporal autocorrelation.
We generated reduced representation libraries (RRLs) from 17 grapevine DNA samples (10 cultivated V. vinifera varieties, 6 wild Vitis species and the reference genome (inbred Pinot Noir) – see Table S1 for details on samples) by digesting each sample with the restriction enzyme HpaII, which has proved useful in the generation of RRLs by others [26], [27].
This conceptual framework may be useful in the generation of testable hypotheses, and provide a 'starting point' for researchers from a variety of different disciplines.
We expect this model to be most useful in the generation of null models in the analysis of biological, technological, and social networks.
This study serves as an illustrative example for those interested in performing space-time cluster analysis of diseases without well-known etiologies and this approach can be useful in the generation of new hypotheses.
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With internal alkynes, we generated synthetically useful regioselectivities in the generation of the α-oxo gold carbene moiety, which enables expedient formation of versatile enone products.
Molecular property prediction is becoming a useful tool in the generation of libraries of molecules with the correct parameters to be useful drug candidates.
The coefficient matrix of each set of equations is independent of rail roughness and therefore this approach is particularly useful in modelling the generation and growth of rail roughness of short wavelengths.
This provides a proof-of-concept that genetic manipulation of HESC can provide a useful tool in the generation of insulin-secreting cells from HESC.
These results demonstrate the fusion of complex disulfide stabilized protein domains, and in conjunction with the numerous pairings tested, demonstrate that sortase A-mediated protein-protein fusion is a useful tool in the generation of fusion proteins when conventional genetic fusion fails.
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