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This system may prove useful for the expression and isolation of mutant myosins associated with skeletal muscle diseases and cardiomyopathies for their biochemical and structural characterization.
The liver stage-specific promoter described in this study might be useful for the expression of such proteins.
Cell-free protein expression systems, such as reticulocyte lysates, are useful for the expression of mammalian proteins, however the protein yield obtained by these methods is usually not sufficient to generate bait columns.
Transient agroinfiltration was useful for the expression of all of the recombinant proteins tested.
Due to the expression of the Pdf2.1: DsRed marker in syncytia, the vector is especially useful for the expression of a gene of interest in syncytia.
Specifically, N. crassa has been shown to be useful for the expression and purification of a functional lignocellulolytic enzyme that can be used in the biofuel industry, where much research is aimed at using cellulosic materials to produce alcohol or other carbon-based fuels (Sun et al. 2011).
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The strategy used for P14K expression in this study may be useful for the heterologous expression of other difficult expression proteins.
On the basis of the present findings, we propose that a system for long-distance transport of artificial siRNA like the one used here would be useful for regulating the expression of specific genes in crops.
We show that enhancers are readily detected with our system and that this technology can be used to create lines of mosquitoes with patterns of Gal4 expression particularly useful for regulating the expression of transgenes in cells and tissues relevant to the study of mosquito/parasite interactions.
Thus, they specifically target the genetic basis of cancer and are particularly useful for inhibiting the expression of oncogenes the protein products of which are inaccessible to small molecules or inhibitory antibodies.
In addition to a set of standard cassettes (Table 1), we have generated a set of cassettes that should prove useful for studying the expression of proteins that are secreted, contain transmembrane domains or localize to other specialized subcellular compartments.
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