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Overall, in previous studies, ctDNA has already been reported to be useful for quantitation of tumor burden in response to surgery, treatments or as a measure of overall survival in a number of malignances, such as colorectal (Leary et al, 2010), breast (Dawson et al, 2013), ovarian and lung cancer (Murtaza et al, 2013).
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However, it also shows that there are some key areas, such as inconsistencies in the procedures used for quantitation, that investigators may want to probe further to assure that the highest quality and most useful studies are performed.
The standard calibration technique (SCT) was used for quantitation.
DPV was used for quantitation of hydrazine in water samples by the standard addition method.
The data were recorded, processed and used for quantitation of individual compounds with Xcalibur® software.
ImageJ (NIH, Bethesda, Maryland) was used for quantitation.
Areas of highest cellularity were used for quantitation.
The Comparative CT method (Applied Biosystems) was used for quantitation.
The label free quantitation (LFQ) algorithm in MaxQuant was used for protein quantitation.
FAME mix C8-C24 (St . Louis Louis MO, USA) was used as the standard for quantitation.
Two deuterated phthalates were used as internal standards for quantitation.
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