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Although useful for proof of principle studies like presented here, unmodified LPEI is not the ideal carrier for targeting transgene expression to the tumor after systemic injection: In our experiment presented here, the level of luciferase activity in tumors strongly varied between individual animals (CMV driven plasmid: 4 high, 2 low, AFP driven plasmid: 3 high, 3 low).
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Using pre-treatment before the bacterial challenge is arguably a limitation of our studies in the primary pneumonia model; this approach is useful for proof-of-principle but does not mirror likely clinical usage.
In an MCI stage, a composite that considers cognitive, functional, and global items is likely to give the best chance for optimal measurement of decline, reflects a global approach to the disease and would be particularly useful for proof-of-concept studies.
Clearly defined post-traumatic OA in humans based on evaluations by MRI and other sensitive imaging approaches may represent a useful, more adapted clinical cohort for proof-of-principle for human trials.
It should be noted that this wavelength is not ideal for imaging in vivo but is useful for these proof-of-principle studies.
The approach we describe is especially useful for trials in rare disorders, or for proof-of-concept studies, where it is important to minimise the trial duration and financial costs, particularly in single-arm cancer trials commonly associated with expensive treatment options.
The following lemma is cited from [17, 18], which is useful for the proof of our main result of this paper.
The following lemmas will be useful for the proof of the main results.
The following lemmas are useful for the proof of the main results.
The following proposition is useful for the proof of the main result.
The following two theorems will be useful for the proof of our results in this article.
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