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The high-copy-number pEXP3-DEST plasmid vector, which has a strong T7 promoter, is designed for in vitro protein production although it is also useful for expression in E.coli strains expressing the T7 RNA polymerase.
This revealed that both approaches generated a similar amount of differentially expressed clones suggesting that both techniques are equally useful for expression profiling.
These results suggest that the polycistronic expression system may be useful for expression of recombinant sTRAIL and improving its potential in cancer therapeutic applications.
Other databases including OryzaExpress (Hamada et al.[2011]), RicePLEX within the Plant Expression Database (PLEXdb) (Dash et al.[2012]), Bio-Array Resource for Plant Biology (BAR) (Toufighi et al.[2005]) and Yale Virtual Center for Cellular Expression Profiling of Rice (Jiao et al.[2009]) are useful for expression pattern analysis of rice genes.
This might be useful for expression studies of genes and their regulatory elements.
The C. reinhardtii HSP70A promoter and the V. carteri hsp70A/ rbcS3 tandem promoter have already proved useful for expression of the aphVIII gene in V. carteri, C. reinhardtii, G. pectorale and E. elegans[ 11, 20, 30].
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A total of 176 phrases were suggested in three textbooks as being useful for expressions for speaking in small groups.
It is useful for small expression matrices, where nonlinear expression relationships between sets of coexpressed genes can be detected although these expression relationships have high entropy.
The transcript sequence and expression polymorphisms are also useful for eQTL (expression QTL) analysis to identify DNA variants that change expression levels and patterns of genes [ 68].
A similar strategy was used to construct pUAS-K10attB that is useful for gene expression in the female germline using the UAS-Gal4 system.
The T7-expression system has been very useful for protein expression in Escherichia coli.
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