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Note that the newly created 6xHis-Venus-GST fusion cassette is itself a useful affinity tag that additionally could be used to estimate protein expression greater than ~1 nM as fluorescence intensity from the Venus domain is linearly proportional to target protein concentration.
All this reflected the fact that grooms – who had, until recently, often been slaves – developed a useful affinity with these flighty animals.
The VNAR domain displayed biologically useful affinity for recombinant and native HBeAg, and recognised a unique conformational epitope.
Critical to the success of a panning experiment is the complexity of the library: the greater the diversity of clones within the library, the more likely the library contains sequences that will bind a given target with useful affinity.
It is noteworthy that, although the sequence space created by the αRep randomization scheme is very large and far from being totally explored in the experimental library, the selections did produce a number of distinct interacting proteins with a useful affinity for practical applications.
It is possible that, when scaled up to a column format, problems with low binding and elution may be resolved, and ATP-coupled sepharose beads may represent a useful affinity purification procedure for P2X receptors, but we decided to proceed with other methods to purify DdP2XA trimers.
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Rationally designed GAG-binding peptides can be useful as affinity vehicles for targeting drugs to the vascular ECM.
The presence of the newly identified AVB-binding epitope will be useful for affinity resin selection for the purification of novel AAV serotypes.
Epitope tags are also useful for affinity isolation of the protein complexes by immunoprecipitation.
Our previous experience using other constructs containing His6 tags is that this tag is not capable of solubilizing improperly folded recombinant proteins, but is useful for affinity purification.
Alternatively, these molecules might be useful as affinity reagents with which to purify the autoantibodies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com