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For the estimation of the in vitro cytotoxic potency of the investigated extracts, an established microtiter plate assay [ 9] was used with three human cancer cell lines: one lung cancer (A-427), one urinary bladder cancer (5637) and one breast cancer (MCF-7) line.
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The breathing-sound experiment was repeated with four human listeners using exactly the same experimental stimuli and paradigm.
We used three human colon cancer cells with distinct degree of differentiation, according to their grade of infiltration or metastasis, in an attempt to represent the variety of cellular changes occurring in precancerous lesions as well as manifest tumors.
BlackBerry PlayBook With seven billion humans now cluttering up the world, National Geographic has released an iPad app outlining some of the implications of population growth, using videos and interactivity.
The synthesized first-strand cDNA fragments were PCR-amplified using two human SOD1-specific primers with an anchored EcoRI or XhoI restriction site, respectively (SOD1EcoRIf: 5′-cagtgaattcgaccagtgaaggtgtggggaagc-3′, SOD1XhoIr: 5′-gatcctcgagccacctttgcccaagtcatctgc-3′).
Test loads were applied and strains were measured with and without an abductor force simulation, using six human cadaveric femora.
For the testing in this study, we used three pooled human CSF samples with different levels of F2-IsoPs and F4-NPs, which were designated as L-CSF, M-CSF, and H-CSF for low levels, medium levels, and high levels of these compounds, respectively.
To design the ultra-sensitive machines, Varna and company used three layers of human improvisation/interaction with choreographer Wayne McGregor's processes in mind: .
Ten fresh frozen human below-knee specimens (five left, five right) were used with appropriate consent of the relatives.
We used a panel of seven human head and neck cell lines with a wide range of EGFR expression representative of that found in human tumours.
The aim of this study was two-fold: firstly, to explore the sequence-dependent cytotoxic effects of combining ZD1839 with radiation using a panel of six human head and neck squamous cell carcinoma cell lines; and secondly, to investigate the sequence-dependent cytotoxic effects of combining ZD1839 with cisplatin and/or 5-FU on two of the cell lines from the panel.
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