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To check that IMP-L2 protein could, in principle, be secreted from the transgene that we used, we increased the level of induction of hsGAL4 by incubating the flies at 37°C for 2 h.
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Every time we use antibiotics, we increase the chance for resistant strains to develop.
As the D A D study does not collect waist circumference, we used increased BMI to reflect this component of the metabolic syndrome, as in other studies (17).
We used increased GBM width as our primary renal end point, since increased GBM width has been shown to be the earliest structural indicator of progression of nephropathy to microalbuminuria in the overall NHDNS study population (29).
We recruited controls that had a tuberculin reaction >15 mm of induration: by using this cut-off, we increased the specificity of the test [19], [20].
Using partial plastic compression we increased collagen densities by up to 10 times.
To minimize those differences, we used an increased LA/Ao ratio as an inclusion criterion.
First we used the increased density of interrogated SNPs from conducting imputation with Bimbam [ 25] as previously described [ 17].
As the amount of data we put into the various systems we use increased, these automated behaviors became more accurate and more helpful.
As technological advances facilitate citrate use, we anticipate increased use.
Using this approach, we increase the sensitivity of the biosensor, resulting in a three times lower LoD.
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CEO of Professional Science Editing for Scientists @ prosciediting.com