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Spermatozoa quality variables were best preserved when the highest SP concentration (50%) was used (viability 97.3%, plasma membrane integrity 98.4%, mitochondrial membrane integrity 36.2%, DNA fragmentation 11.1%).
This study points out the great need for thorough characterization of cell nanoparticle interactions as subtle time-dependent effects are hard to monitor and commonly used viability and functionality assays are not sufficient to address the broad spectrum of possible interferences of the nanoparticle with normal cell functioning.
Many of the earliest studies into the genetic control of autophagy used viability during starvation as an assay to screen for autophagy mutants in yeast [2,4 7].
Thus, the SYBR Green I/PI assay provides a convenient and more sensitive assay for evaluating the antibiotic susceptibility of B. burgdorferi than other currently used viability assays, such as the LIVE/DEAD BacLight method, and it can be used for high-throughput screening of new drugs.
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"Developers have been using viability for centuries, it's their bread and butter," says Pat McAllister.
"Developers have been using viability wizardry for too long to dodge their obligations.
We examined a wide range of soils using viability PCR based on the photoreactive DNA-intercalating dye propidium monoazide4.
Fittipaldi, M., Nocker, A. & Codony, F. Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification.
In this paper, we use viability tools to design Lyapunov functions for nonlinear state-dependent impulsive dynamical systems : the main idea is to partition the continuous state space.
The compromise decision of the Court was to use "viability" as the line after which a state might limit the right to an abortion based on the compelling interest of fetal life.
Interestingly a similar situation of apparent redundancy, using viability in S. cerevisiae in rich media as the assay, is found for FG-NUPs.
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